This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh tissue or cells.
The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length.
Introduction to Nucleic Acid Electrophoresis Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography Introduction to Southern and Northern Blotting The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting.
This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample.
After adding chloroform or 1-bromo-3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins.
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